DEVELOPMENT AND VALIDATION FOR SIMULTANEOUS ESTIMATION OF CEFUROXIME AXETIL AND LINEZOLID IN BULK DRUG AND PHARMACEUTICAL DOSAGE FORM BY RP- HPLC
Journal: Indo American Journal of Pharmaceutical Sciences (IAJPS) (Vol.04, No. 10)Publication Date: 2017-10-06
Authors : Naveen Kumar G S; Srinivasa U;
Page : 3567-3571
Keywords : Cefuroxime axetil; Linezolid; RP-HPLC and Validation;
Abstract
A new sensitive, accurate, precise and validated RP-HPLC method was developed for the concurrent assessment of cefuroxime axetil and linezolid in bulk drug and pharmaceutical dosage form. The wavelength selected for quantitation was 276 nm. The method has been validated for linearity, accuracy, precision, robustness, limit of detection and limit of quantitation. Linearity was observed in the concentration range of 2‑12 µg/ml for cefuroxime axetil and 6‑36 µg/ml for linezolid. For RP‑HPLC, the chromatographic separation was achieved by systronics C18 (250×4.6 mm) 5 µm column using phosphate buffer (pH 7): methanol (60:40 v/v) as mobile phase with flow rate 1 ml/min. The retention time of cefuroxime axetil and linezolid were found to be 3.127 min and 11.986 min, respectively. The developed method was simple, specific and economic, which can be used for simultaneous estimation of cefuroxime axetil and linezolid in tablet dosage form. Keywords: Cefuroxime axetil, Linezolid, RP-HPLC and Validation
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