Pro- and Antiphlogistic Concentration of Cytokines in Rats’ Serum after Long-Term Administration of Omeprazole and the Simultaneous Injection of Multiprobiotics and Omeprazole

Nowadays, the literature data about the role of cytokines in stomach and intestines phlogistic processes develops against the backdrop of long hypoacidity of gastric juice in the absence of H. pylori infection is limited and applies only to certain cytokines. The aim – to explore the balance of pro- and antiphlogistic cytokines in the rats blood serum during long hypoacidity of gastric juice and the impact of multiprobiotics. Objects and methods. The research conducted on nonlinear white male rats with an initial weight 160-180 g, divided into four groups with 10 animals in each. Rats in group I served as control objects. They were intraperitoneally (i/o) administered 0.5 ml of water for injection within 28 days. The rats in Group II were daily intraperitoneally (i/o) injected omeprazole (produced by "Sigma-Aldrich" USA) at a dose of 14 mg/kg, dissolved in 0.2 ml of water for injection. The animals of the third group were once a day during 28 days administered multiprobiotic "Symbiter® acidophilic" concentrated (Symbiter together and omeprazole). Animals of the fourth group were administered omeprazole together with multiprobiotic "Apibakt® once a day during 28 days. "Multiprobiotics Symbiter and Apibact (produced by SPC "O.D. Prolisok", Ukraine) were administered together with omeprazole n/a dose of 140 mg/kg (1,4 * 1010 CFU/kg). Results and discussion. As a result we identified that after 28 days of administration of omeprazole concentration of pro-inflammatory cytokine IFN-γ increased by 58,5% (p <0,05). The group of rats given the long-term co-administration of omeprazole and multiprobiotic "Symbiter" showed IFN-γ concentration in the serum at the level of 30,8% (p <0,05) which is lower in comparison with the group of rats which were administered omeprazole alone. After a 28-day co-administration of omeprazole and multiprobiotic "Apibact" IFN-γ concentration in the serum of the rats was at 24,6% (p <0,05) which is lower in comparison with the group of rats administered one of omeprazole and 19, 5% (p <0,05) higher than the figure in control. After 28 days of administration of omeprazole in rats increased serum concentration of TNF-α. This increase was 73,3% (p <0,05). The group of rats given the long-term co-administration of omeprazole and multiprobiotic drugs "Symbiter" and "Apibact" concentrations of TNF-α showed the rats serum decrease to 15,4% (p <0,05) and 17,9% (p <0,05), respectively, compared with the group of rats, which were injected only omeprazole during the same time. Compared with the control group of rats, the rats administered both omeprazole multiprobiotics "Symbiter" and "Apibact" during 28 days indicated that the concentration of TNF-α in serum of the rats serum remained respectively 46,7% (p <0,05) and 42,2% (p <0,05) higher. After 28-day administration of omeprazole to rats the concentration of pro-inflammatory cytokine IL-1β increased 80,2% (p <0,05) compared with the control group. After a 28-day co-administration of omeprazole and rats multiprobiotic "Symbiter" concentration of IL-1β in serum decreased to 22,5% (p <0,05) compared with the group of rats which were administered one of omeprazole and by 39,6% more (p <0,05) compared with the concentration of IL-1β in the serum of the rats in the control group. The rats injected multiprobiotic "Apibact" and omeprazole during 28 days displayed the concentration of IL-1β in serum at 40,5% (p <0,05) which is lower than the group of rats administered only omeprazole, and not significantly different from the rate of the control group rats. After 28-day administration of omeprazole the concentration of anti-inflammatory cytokine IL-4 in the rats serum was reduced by 39.8% (p <0.001) compared with control group rats. Adding to omeprazole multiprobiotic "Symbiter" or "Apibact" prevented the decrease in the concentration of IL-4 in the rats serum.