Study of the Activity of Phospholipase A2 in Venom of the Transcaucasian Macrovipera Lebetina Obtusa
Journal: International Journal of Environment, Agriculture and Biotechnology (Vol.2, No. 4)Publication Date: 2017-07-02
Authors : Shafiga Topchiyeva Elmar Babayev Huseyn Abiyev;
Page : 2102-2107
Keywords : phospholipase A2; venom; snake; Macrovipera lebetina obtusa; heavy metals; enzyme activity.;
Abstract
The activity of phospholipase A2 in the venom of the Transcaucasian Macrovipera lebetina obtusa, collected from viperas inhabiting in different regions of Azerbaijan in terms of pollution, was studied. The lowest (30,2 IU / mg) was detected in the venom of Viperas collected in Sabirabad district, Karatuga village and the highest was detected in the venom of Viperas collected in Baku, s. Bina (38,5IU / mg). As a result of experimental studies it follows that the activity of phospholipase in the venom collected in the vicinity of the Sabirabad district Karatuga village and Agsu district Garagoyunlu village, Gobustan district Childag village, Bina and Sumgait is 30.2 ± 1.1 IU / mg, 32.6 ± 0.9 IU / mg, 34.5 ± 0.8 IU / mg, 38.5 ± 0.2 IU / mg and 36.1 ± 0 , 8 IU / mg, respectively. Thus, the activity of phospholipase A2 in the venom of the Transcaucasian Viperas Macrovipera lebetina obtusa collected from snakes inhabiting different in the degree of contamination of the regions of Azerbaijan was studied. The results of the experimental data can be used to determine the biological activity of the venom samples, including, for identification and standardization of the venom of the vipera.
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