STUDY OF SINGLE NUCLEOTIDE POLYMORPHISM (SNP) AND GENE EXPRESSION OF PTEN IN IRANIAN GASTRIC CANCER PATIENTS

Gastric cancer is recognized as the fourth most common cancer in the world, and is the second leading cause of cancer death. PTEN is a tumor inhibitor gene. PTEN mutation is observed in most of the cancerous malignancies. In this study, the expression and common PTEN gene polymorphism in blood samples of patients with gastric cancer were studied using REAL TIME PCR and RFLP PCR techniques in Iranian population. 45 blood samples (36 patients and 9 controls) were studied in this study. After extracting the RNA and confirming its quality, cDNA synthesis was performed and the expression of the gene was evaluated by qPCR. In order to investigate the single-nucleotide change in rs10490920, the DNA was extracted from the specimens and then PCR was performed. After obtaining ct, the samples RQ was analyzed and analysis of samples RQ showed that the PTEN Gene expression was reduced in patients compared with controls. In patients with rs10490920 polymorphisms in the PTEN gene, the risk of the disease increased. The results of the study indicate a reduction in gene expression in cancer patients. Thus, it may be possible to use Pten gene expretion assesment as a possible biomarker in early diagnosis or in the treatment process. Keywords: Gastric cancer, PTEN, gene expression, polymorphism, Real time PCR