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PRODUCING OF BACTERIAL CONCENTRATES WITH HIGH CHOLESTEROL LOWERING ACTIVITY

Journal: "Foods and Raw materials" Journal (Vol.4, No. 1)

Publication Date:

Authors : ;

Page : 27-35

Keywords : bifidobacteria; propionic bacteria; lactobacteria; cholesterol-lowering activity; exopolysaccharide; bacterial concentrate;

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Abstract

The screening of 8 probiotic strains (Propionibaсterium, Lactobacillus and Bifidobacterium) characterized by high cholesterol-lowering activity was carried out in the scientific research laboratory of East Siberia University of Technology and Management (Ulan-Ude, Russia). In order to research biotechnological potential of probiotic microorganisms and to conduct statistical analysis we used a mix of standard and modern physicochemical, biochemical and microbiological methods. The strains Lactobacillus helveticus 3, Propionibacterium shermanii AC-2503 and Bifidobacterium longum DK-100 were found out to have the highest cholesterol-lowering properties. It can be seen that cocultivation of Lactobacillus helveticus 3and Propionibacterium shermanii AC-2503 strains shows high population density, cholesterol-metabolizing, antimutagenic, adhesive properties and exopolysaccharide biosynthesis. It indicates stable symbiotic bonds between cultures, which served as the basis for making a combined ferment. The analysis of biotechnological potential of Lactobacillus helveticus 3 and Propionibacterium shermanii АС-2503 strains and the study of organoleptic, physicochemical, probiotic properties and cholesterol metabolizing activity of the combined ferment have allowed to establish an optimum culture ratio in the ferment - 5 : 95. Optimum cultivation conditions of the continuous culture Lactobacillus helveticus 3and the combined ferment were selected, process parameter of biologically active supplement and frozen ferments of direct loading were substantiated.

Last modified: 2017-11-28 17:24:46