ELECTRON MICROSCOPIC STUDY OF RAT HEPATOCYTES IN THE DYNAMICS AFTER INTRAPERITONEAL INJECTION OF HIGH DOSE OF METHANOL

Wistar rats were studied electron-microscopically hepatocytes (SE) of the liver in the dynamics, after 25 and 40 minutes, 1, 3, 7 and 14 days after a single intraperitoneal injection of methanol of 2.5 g / kg body weight of the animal. It is shown that in 25 - 40 minutes in the GP notes focal disorganization of ultrastructures in the cell cytoplasm. After 1 day revealed the pathology of mitochondria cristae show signs of degradation or complete absence, there is looseness mitochondrial membrane and is determined by reducing the amount of glycogen granules. After 3 days in the SE part of the notes of varying degrees of disruption and destruction of the ultrastructure, there are signs of intranuclear and cytoplasmic swelling with destruction of cytoplasmic organelles. Themarked swelling of mitochondria intermitohondrialnichristie and the destruction of the formation of vacuoles. In the cytoplasm, ZES pronounced narrowing of tubular membranes with degranulation, the almost complete absence of glycogen granules. Plasmolemma cells loose and fragmented. By 7 days in terms of GP pronounced polymorphism alterative changes in the cytoplasm from intracytoplasmic edema lesions or total cytoplasmic homogenization mitochondria and destruction of elements of granular endoplasmic reticulum to the cell intact and ultrastructure of cells with signs of compensatory and regenerative processes. Around the organelles are diffusely dense single or merging small groupsosmiophil glycogen granules. On the 14th day marked as a GP with normal ultrastructure, and with signs of destructive changes. Methanol, in the first place causes changes in the ultrastructure of the mitochondria, indicating that early Abuse energy processes in the liver cells, as well as changes in the number and structural characteristics of glycogen granules, indicating that the toxic effect of a substance on carbohydrate metabolism in the liver. Data presented in the article are the initial stage of investigation of the influence of methanol on the tissues оf rat. Perhaps a more advanced and in-depth study of the impact of this toxic factor on the living organism experimental animalswill allow to introduce the structural mechanisms of action of methanol on various human organs and systems.