Dynamics of Indexes of White Blood Cells during Experimental Biliary Peritonitis

One of the most serious diseases of the abdominal cavity organs is biliary peritonitis. The aim of the research is to study the leukocyte index of intoxication and the number of leukocytes in experimental gallstone in animals that underwent complex abdominal sanitation. Materials and methods. The study was performed on 180 rats of the Wistar line weighing 180-200 grams. Animals were divided into 4 groups: Group 1 – group of intact rats (20 animals). Group 2 – control group – rats, who modeled biliary peritonitis without further correction (80 animals). Group 3 – rats whose simulated biliary peritonitis was corrected by abrasion of the abdominal cavity with a solution of furacilin (1: 5000) with further use of standard antibiotic therapy. Group 4 – rats whose simulated biliary peritonitis was corrected by double sanitation. The first sanitation is detoxification with 0.04% sodium hypochloride solution 12 hours after the second injection of bile. The second sanitation is a mixture consisting of decametoxin compound (10 mg / 50 ml solution), sodium hyaluronate (250 mg / 50 ml solution), and succinate buffer 6 hours after the first sanitation. Biliary peritonitis was modeled as follows: a sterile 10% solution of calcium chloride (1 mg / 100 g of body weight) was administered intramuscularly. There was a focus of aseptic inflammation. After 72 hours, intraperitoneal bile was injected twice with 0.33 ml / 100 g of body weight at intervals of 12 hours. Blood sampling from the tail vein was performed at the end of the 1st, 3rd and 7th day of the BP model simulation. Results and discussion: 1. Analysis of leukocyte dynamics. On the 1st day, very significant differences (at a significance level of p <0.001) between the results of all the study groups were revealed. The second group is characterized by the maximum increase of the number of leukocytes. The difference in the analysis of the index of the 4th group was revealed not only in comparison with the 1st and 2nd groups, but also in comparison with the 3rd group, which indicates the greater effectiveness of the method of abdominal sanation proposed by us already for 1 day. Differences between the results of the 4th and 3rd groups are 92.6% (the results of the 4th group are closer to the norm). On day 3, a similar pattern was observed in the analysis of each group of animals. The difference between the results of the 4th and 3rd groups was 81.3%. On the 7th day of the experiment, a decrease in the number of leukocytes was found below the normal level (on the 1st and 3rd days, their increase was noted). The differences between groups 3 and 1 were at the level of significance p <0.01, and between 4 and 1 – at the level of p <0.001. 2. During the investigation of the LII, the following data were obtained: On the first and third day of the study, the differences between the 1st, 2nd, 3rd and 4th groups are very significant (p <0.001). The maximum increase was found in the group with experimental HP without correction. At the same time, the results of the 4th group testify to a decrease in LII on the background of the proposed sanitation, and are closer to the results of intact animals compared to group 3 (by 80.8% for the first day, and 69.6% for the 3rd). At the 7th day, LII decreased in the 3rd and 4th groups as compared with the values of the indicator in intact animals). In this case, differences were found only between 4 and 1 groups, but already at the significance level p <0.05. Conclusion: During the analyzing the level of leukocytes in the blood of rats, there is a more pronounced correction of the indices in the 4th group as compared to the third, on the 1st and 3rd days of the experiment.