ASSESSMENT OF IN VITRO NARINGENIN RELEASE FROM SOLID LIPID NANOPARTICLES AND KINETIC MODEL PROFILING: APPLIED ULTRAVIOLET-VISIBLE SPECTROPHOTOMETER

A new, simple, specific, rapid, precise, highly accurate, reproducible and cost effective Ultraviolet-Visible spectrophotometric method was developed and validated, according to the International Harmonization Guidelines, for the determination of naringenin from solid lipid nanoparticles. Absorption maximum of Naringenin was found to be at 287.49nm in methanol. The linearity range was found to be 5-25μg/mL with high correlation coefficient value of 0.999. The detection and quantification limits were found to be 0.1879μg/mL and 0.5694μg/mL, respectively. This method was shown to be specific, selective, precise at the intra-day (relative standard deviation less than 0.7046%) and inter-day (relative standard deviation less than 1.5424%) level and accurate with recoveries between 98.77-100.43% (relative standard deviation less than 0.3924%). Method robustness observation indicates that method was robust. The suitability of the method for naringenin quantifications was assessed by the determination of entrapment parameters and by studying the naringenin release profile from SLNs. High entrapment efficiency (91.922 ± 0.717%) and drug loading (3.506 ± 0.027%) were observed. Kinetic models (zero order, first order, Higuchi, Hixson-Crowell, Korsmeyer-Peppas and Baker-Lonsdale) were used to fit the obtained release profile and to predict the in vivo performance of naringenin-loaded SLNs. An anomalous non-Fickian transport was found, which indicate a controlled drug release system.