HPTLC Fingerprint Profile of Petroleum Ether Extracts from Wasteland Herb Cassia tora L.

Cassia tora L. (Family: Caesalpiniaceae) is a wild herb generally distributed in tropical countries. In India, it grows as a wasteland rainy season weed. Traditionally, Cassia tora L. is used for treating various ailments like leprosy, ringworm infection, ulcers and many more. The aim of the study is to develop a HPTLC fingerprint profile of petroleum ether extracts from aerial parts of Cassia tora. The HPTLC method was developed for the separation of phytoconstituents present in petroleum ether extracts using mobile phase of n-Hexane: Ethyl acetate: formic acid: Acetic acid (60:40:2.5:2.5) and scanned under UV at 254 nm, 366nm and under white light. HPTLC fingerprinting of leaf extract displayed 10 constituents, seed (7 constituents) and flower (11 constituents). The HPTLC fingerprinting profile for petroleum ether extract may provide appropriate identification and quantification of non polar marker compounds and in the authentication of this plant.