COMPARATIVE EFFECT OF CRUDE EXTRACT OF PARKIA BIGLANDULOSA AND ITS ISOLATE ON REGENERATIVE ANGIOGENESIS IN ADULT ZEBRAFISH MODEL

As cancer continues to be one of the biggest threats worldwide and causes maximum deaths after cardiovascular diseases, a greater emphasis must be given for newer strategies for the development of anti-cancer drugs. Cancer treatment strategies target various mechanisms such as apoptosis, metastasis, angiogenesis, etc. Angiogenesis, which is the process of generation of new capillary blood vessels, can be an excellent target for anti-cancer therapy, as a tumor cannot grow further without it. The present study aims to evaluate and compare anti-angiogenic activity of ethanolic extract of Parkia biglandulosa (EEPB) and its isolate β-sitosterol by regenerative angiogenesis assay using caudal fin of adult zebrafish. One of its active constituents that is β-sitosterol, which was expected to show anti-cancer activity, was isolated by using flash chromatography and its activity was checked along with crude ethanolic extract of Parkia biglandulosa (EEPB). Results of regenerative angiogenesis compared by one way ANOVA followed by Tukey's test showed significant inhibition of fin growth when compared with control group which signified anti-angiogenic effect of test compounds.