Scanning Electron Microscopic (SEM) Aspects of Malassezia Pachydermatis Isolated From Dogs

Dogs presented with the signalment of pruritus, erythema, alopecia, eczema and any visible skin lesions were considered and skin sample was collected using sterile cotton swabs / adhesive tape. The sample was transferred to the glass slide and then heat fixed and stained with new methylene blue stain, for confirmation. Later the sample was processed and subjected for the scanning electron microscopic procedure. The Malassezia pachydermatis blue coloured with foot print or peanut shape. Electron microscopically, the yeast colonies grown on Sabourauds dextrose agar were raised, doomed or high convex and smooth with cream colour. The organism was measured between 3.16 to 5.29 microns length, which appeared as globose to ellipsoidal in shape, round at one end and blunt at the other, which was associated with asexual reproduction.