BONE REGENERATION IN CRITICAL-SIZE CALVARIAL DEFECT IN RATS USING INNOVATIVE NANO COMPOSITE MATERIAL OF CHITOSAN / NANOHYDROXYAPATITE COMPOSITE

Healing of standardized critical-size calvarial defects in rats was performed with nano composite material of nano-hydroxyapatite/3 % solution of chitosan in citric acid, and electrospinning fibres of poly lactic acid. Histologically at the 84 day, the implanted rats exhibited full closure of the defect by new developed connective and osteoid tissues penetrating in reticular pattern within the implant components. These results indicate that by using a newly created composite paste critical cranial defects can be successfully retrieved and probably is also suitable for other cases in bone augmentation surgeries.