Fluorescence Report on the Binding Interaction of Imidazole using Bovine Serum Albumin

Nuclear Magnetic Resonance (NMR) spectra, mass and Carbon Hydrogen and Nitrogen (CHN) analyzer synthesize and characterize bioactive Imidazole Derivatives (ID). Fluorescence and ultra violet– visible absorption spectrometry aids in investigating ID and bovine serum albumin. Imidazole–Bovine Serum Albumin (BSA) complex leads to BSA fluorescence quenching, in where ID performs the BSA quenching. Then the calculation of binding constant is made based on quenching analysis. Forester's nonradiation energy transfer determines the binding distance between bovine serum albumin and imidazole, where the influence of certain ions over the binding constant between them has also been carried out.