SYNTHESIS AND ANTIMYCOBACTERIAL ACTIVITY OF BENZIMIDAZOLE DERIVATIVES

In the present study, the objective is to synthesize novel compounds from the benzimidazole moiety,recognized for its various biological potentials. Chalcone being a very good synthon was utilized to combine with the benzimidazole moiety to design agents with significant antimycobacterial activity. Benzimidazole is formed when o-phenylene diamine is heated with organic acid i.e. formic acid; inpresence of strong alkali sodium hydroxide it will gives benzimidazole. Benzimidazole was acetylated with acetyl chloride, the intermediate substituted chalcones were synthesized from acetylated benzimidazole,in order to synthesize different substituted aromatic aldehydes of the basic moiety, 1-sub. benzimidazole to obtain the different benzimidazole derivatives. Their structures were characterized by various spectral techniques like IR, NMR and MS. For the preliminary screening (antibacterial and antifungal)for antimicrobial activity, the test was performed by disc diffusion assay and determination of minimum inhibitory concentration (MIC) by broth dilution method. The antimycobacterial screening was performed against Mycobacterium tuberculosis by Almar blue assay (ABA) and Luciferase reporter phase (LRPA)techniques using isoniazid as the standard.