EFFICIENCY OF COMBINED APPLICATION OF L-ARGININE AND NUCLEAR FACTOR Β κ B INHIBITOR IN CORRECTING CONNECTIVE TISSUE DISRUPTION IN THE SKIN OF RATS UNDER EXCESSIVE SODIUM NITRATE INTAKE

Objectives: to investigate the effects produced by the combined action of L-arginine and the NF-κB inhibitor ammonium pyrrolidine dithiocarbamate (PDTC) on the markers of connective tissue disruption in the skin of white rats and its biomechanical properties under excessive intake of sodium nitrate. Material and methods. The studies were performed on 35 white Wistar rats. We assessed the concentration of collagenolysis and proteoglycans depolymerization products (free oxyproline, hexuronic acids) in the skin homogenate, as well as dermal tensometric characteristics. Results. It has been found out that a 30-day administration of sodium nitrate is accompanied by the following changes in the skin tissues: enhancement of the processes of collagenolysis and proteoglycans depolymerization, impairment of biomechanical skin properties (strength and elasticity). It has been shown that the combined administration of L-arginine (500 mg/kg) and PDTC (76 mg/kg) once every three days starting from the 15th day of nitrate intoxication, demonstrates much more pronounced effects in reducing the concentration of free oxyproline and hexuronic acids in the skin homogenate and improving skin tensometric indicators as compared with their separate administration. Conclusions. The combined effect of L-arginine and PDTC as a selective NF-κB inhibitor under nitrate intoxication is more effective than the isolated application of these substances. Their combined application limits the connective tissue disruption in the skin and improves its biomechanical properties.