GREEN SPECTROPHOTOMETRY AND SPECTROFLUORIMETRIC METHODS FOR RAPID ECONOMIC DETERMINATION OF TRAVOPROST: STUDY ON INCLUSION COMPLEX WITH BETA CYCLODEXTRIN

Green spectrophotometry and spectrofluorimetric methods for determination of travoprost(TRAVO) were designed and investigated. Spectrophotometric methods are based on measuring the absorbance of the drug in water and in presence of 1.5 mL of 1.0% (w/v) beta-cyclodextrin (ẞ-CD) at 277 nm. The absorbance concentration plots were rectilinear over the range of, 10 – 50 μg/mL and 2.5 – 30 μg/mL with limits of detections; 0.018 and 0.009 μg/mL in absence and presence of ẞ-CD respectively. The fluorescence enhancement of a highly sensitive spectrofluorometric method is based on investigation of the fluorescence spectral behavior of travoprost in aqueous organized system, ẞ-CD. In aqueous solution travoprost is well incorporated into ẞ-CD, with enhancement of its native fluorescence. The fluorescence was measured at 305 nm after excitation at 277 nm. Fluorscence spectroscopy of the host – guest interaction between travoprost and ẞ-CD shows 1:1 inclusion complex with ẞ-CD, with an association constant of 21.86 M-1 . The quantum yield was 0.16 and 0.20 in absence and present of ß-CD. The fluorescence-concentration plots are rectilinear over the range of 10 – 250 and 2.5 – 35 ng/mL, with lower detection limits of, 0.003 and 0.002 ng/mL respectively. The methods were successfully applied to the analysis of pharmaceutical preparation and the results were in good agreement with those obtained with the official HPLC method. The proposed methods are inexpensive, simple, and sensitive for quality control of the drug in QC laboratories. Keywords: Travoprost, spectrophotometry, spectrofluorimetry, ßeta-cyclodextrin, inclusion complex, validation