ROLE OF ENDOGENOUS INTOXICATION IN ACUTE EXPERIMENTAL PANCREATITIS

The purpose of the work was to determine the patterns of development of endogenous intoxication in the dynamics with acute L-arginine induced pancreatitis. Methods. The article reveals the research results on 65 white male rats of Wistar line weight 180-220 g, on which was modelled acute pancreatitis (AP). Animal blood samples were taken at 12, 24, 48 and 72 hours of AP to determine the content of middle mass molecules (MMM) for the assessment of endogenous intoxication (EI). Results of experiment. It has been found out that in acute experimental pancreatitis there is elevation of MMM2 level at a wavelength of 280 nm (protein component), additionally a massive release of the nucleoprotein component (MMM1 at 254 nm) is observed as well. It indicates the development of endogenous intoxication, which is the most intensive at 24 h of experiment. Conclusion. The results of the study have showed that acute L-arginine-induced pancreatitis is accompanied by intense endogenous intoxication, which has been observed at 12 hours after the experiment and is increasing to 72 hours with the most rapid increase at 24 hours.