ANALYSIS OF INDICATORS OF ENZYME HYDROLYSATES OF FEATHER-DOWN RAW MATERIALS OBTAINED WITH THE USE OF MULTI-ENZYME COMPOSITION
Journal: "Foods and Raw materials" Journal (Vol.5, No. 2)Publication Date: 2017-12-26
Authors : Astakhova L.A. Asyakina L.K.;
Page : 112-120
Keywords : Feather; enzymatic hydrolysis; biosafety; physical and chemical properties; feed supplement;
Abstract
Feeds, feed preparations and high-protein feed supplements are complex multicomponent compositions that, when stored, used, processed and transported, can change their physicochemical properties, microbiological indicators and toxicological properties. In this regard, it is very important to study the above properties and quality indicators. The paper describes a study of the peptide profile, physico-chemical and toxicological properties of the enzymatic hydrolysates of feather-down raw materials obtained during a multifactorial experiment to optimize the conditions for enzymatic hydrolysis of feather-down raw materials. The raw materials were previously subjected to a short-term hydrothermal treatment to improve the course of enzymatic hydrolysis. The enzymes obtained from bacterial organisms were used for the enzymatic hydrolysis of raw materials: Protolade B and Protease 2630#2256. The following results were obtained for the physicochemical properties of enzymatic hydrolysates: the content of protein and solids in the enzymatic hydrolysates of feather-down raw materials varied in the range of 1.80-3.00% and 2.6-4.4%, respectively. In this case, the mass fraction of protein was 67.7-70.6% in terms of the mass fraction of moisture. The mass fraction of ash, in terms of the mass fraction of moisture, did not exceed 0.43% in all the samples of enzymatic hydrolysates, the mass fraction of fat was 0.74%, the mass fraction of crude fiber was 1.28%, the mass fraction of sodium chloride was 1.92% the mass fraction of the impurities insoluble in hydrochloric acid was 0.77%. It has been shown that enzymatic hydrolysates corresponded to TU 9219-094-23476484-09 «Hydrolyzed feed flour. Feather protein concentrate» for their microbiological properties (QMAFAnM, coliforms, pathogenic microorganisms, toxin-forming anaerobic bacteria and bacteria of the genus Proteus) and chemical safety parameters
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