ENHANCING IMMUNE MODULATING PROPERTIES OF HORSE MILK USING PLANT EXTRACT FROM EMINIUM REGELII

The article presents the research results of moisture, tannins and other biologically active compounds (alkaloids, saponins, flavonoids, including luteolin and quercetin) content in tubers of Eminium Regelii. The authors determined the concentration of luteolin and quercetin at which the growth of cancer cells HCT-15 is slowed down by 50 % (IC50). They developed the method of obtaining Eminium Regelii extract and studied its physical and chemical properties. Biologically active substances found in the tubers of Eminium Regelii were also present in the extract. Besides, the concentration of these biologically active substances in the obtained extract increases compared to the Eminium Regelii tubers, whereas tannins content is lower. Moreover, the authors found vitamin C in the obtained extract, which is known for anti-stress effect. Besides, it enhances the protective mechanisms of the body. The authors developed horse milk (or koumiss) production technology using Eminium Regelii extract. A new fermented milk drink is characterized by high consumer properties and can be recommended for mass consumption as an immune modulator for certain population groups with weakened immune system. In terms of safety, koumiss with Eminium Regelii extract complies with the requirements of the regulative documents. On the basis of the carried-out preclinical tests the authors found out that intragastric introduction of immune modulating koumiss with Eminium Regelii extract in dose of 0.1-0.25% wt did not cause marked toxic changes in the physiological, hematological and morphological parameters of the experimental animals. On the contrary, it activated humoral immunity and increased nonspecific phagocytic resistance of the organism, i.e. it raised the immune status of the experimental animals.