ISOLATION AND IDENTIFICATION OF CLOSTRIDIUM PERFRINGENS CAUSING ENTEROTOXAEMIA IN BOVINE OF KACCHI DISTRICT BALOCHISTAN

The causal agent of necrotic enteritis was isolated from the bovine of kacchi district Balochistan. Total 200 fecal samples were collected, out of which 74% samples were positive and 26% were negative for Clostridium perfringens. The age wise distribution was 20 % in 6 month, 16 % in 1 year, 13% in 2 years, 11% in 3 years, 8% in 4 years and 6% in 5 years. However species wise results showed that the cattle were 54% and buffaloes were 20% affected with enterotoxaemia. The season wise result showed that the incidence of enterotoxaemia was more in spring season. Clostridium perfringens was confirmed through different biochemical tests and gram staining. Antibiograms result showed that Clostridium perfringens was sensitive against Chloramphenicol, Penicillin, Tetracycline, Glycopeptides, Quinolones, Lincosamides and Macrolides classes while resistance to Polypeptides, Flagyl and Sulfonamides classes. The animal trail showed that Clostridium perfringens affected the vital organ. The hemorrhagic lesion on lungs, kidneys, and intestine were observed at autopsy. Whereas confirmed by PCR showed clear bands of 233bp of CPE gene. Key words: Clostridium Perfringens, Bovine, Kacchi, Balochistan, PCR.