Bioethanol Production From Sago Metroxylon spp Core by Using Enzyms

Sago (Metroxylon spp ), an endemicplant of Indonesia, ispotential for bio-ethanol base material Bioethanol can be produaedfrom sago core, starch as well as fiber by utilizjng certain enzymes. This research was conductedto determine the effectivenees ef alpha amylase and gluco-amy laseenzymes for bio-ethano prodnaion. The process employed in this research include delignification using alpha amylase enzyme, saccharifi cation using gluco avrylase enzyme,and 3-d~ fermentation attenrperature28-30°C and pH of 5-5.5.the processconcl11dedwithdistillation at 78-10rf C Laboratory results showed thatproducing ethanolfrom sago strach with a combination ef 0. 18 mL/ liter alpha amylase and 0.48 ml/ liter gluco amyiasegives the highestpercentageef bio-etbanol, name!J 6 5%. The combination of 0. 13 mL/ liter alpha anrylase, 0.11 mL/ liter gluco aurylase and 0.33g/ literyeast on sago core only produces 44.84% ethanol using the same combination on sagofiber gives as low as 8.26% ethanol At the small scale, production using stracb, core andfibergives24%, 11% and 4% ethanol respectively.