A Study of Pomegranate Fruit on Cisplatin- Induced Acute Renal Failure in Adult Bulb/C Mice

Aim: Study was undertaken to evaluate the effect of Punica granatum L. on cisplatin-induced renal failure in adult Bulb/c mice. Materials and Methods: Gr.-I rats served as normal, received 0.5 ml of 5% Tween-80 in distilled water, Gr.-II was injected with Gentamicin (100 mg/kg, ip), Gr.-III was injected with Gentamicin and selenium (2 mg/kg, ip), and Gr.-IV-IX were given orally Punica granatum fruit chloroform extract (PGCE) and Punica granatum methanol extract (PGME) at the doses of 100, 200 and 400 mg/kg, respectively, were administered for three days in cisplatin-induced renal failure in adult Bulb/c mice. On last day, Blood and 24h urine was collected and used for estimation of serum and urine creatinine, urea, uric acid levels. The kidney homogenate was used for the estimation of LPO, SOD, CAT and GSH levels and Kidney sections were analyzed for histopathology. Results: Cisplatin caused significantly (p<0.001) decreased in the levels of SOD, CAT and GSH, when compare to normal group of mice. More over treatment with Punica granatum L. significantly increased the levels of SOD, CAT, GSH when compare to control group of mice. The Nephroprotective potential of Punica granatum L. can be attributed to its antioxidant property. Conclusion: It is concluded that administration of PGCE and PGME extracts not only did not ameliorate nephrotoxicity induced by Cisplatin in adult Bulb/C mice, but also aggravated renal damage.