ResearchBib Share Your Research, Maximize Your Social Impacts
Sign for Notice Everyday Sign up >> Login

DETECTION OF MYCOBACTERIUM TUBERCULOSIS DNA IN SPUTUM SAMPLES WITH MPB 64 GENE PRIMERS USING POLYMERASE CHAIN REACTION

Journal: International Journal of Advanced Research (Vol.6, No. 4)

Publication Date:

Authors : ; ;

Page : 1089-1093

Keywords : Tuberculosis; Cough; Ziehl-Neelsen staining; Sputum.;

Source : Downloadexternal Find it from : Google Scholarexternal

Abstract

Tuberculosis (TB) is preventable and curable, but it can lead to death if no actions are taken. In order to prevent transmission, it is necessary to identify infectious TB patients in a timely manner. The present study was aimed to detect the mycobacterium tuberculosis DNA in sputum samples with MPB 64 gene primers using PCR. Hundred patients were enrolled in the study based on inclusion and exclusion criteria. Sputum samples were collected and processed for the required procedure. Ziehl-Neelsen staining was done and slides were examined under microscope. DNA was extracted from the samples and amplified using PCR. Staining results of both spot and morning samples was compared under microscope with grading.

Last modified: 2018-05-26 18:22:20