The Estimation of Mepivacaine Drug in Mepivacaine Hydrochloride Injection by Reverse Phase High Performance Liquid Chromatography

A simple, precise, rapid and accurate reverse phase HPLC method was developed for the estimation of Mepivacaine drug in Mepivacaine Hydrochloride Injection. Develosil ODS-MG-5, (150 x 4.6 mm), 5�m with mobile phase consisting of Potassium dihydrogen phosphate buffer and acetonitrile were mixed in the ratio 65:35 (v/v) respectively and degassed for about 10 minutes. Isocratic mode of chromatography technique was used. The flow rate was 1.5 mL/min and the eluents were monitored at 220 nm. The retention time of the Mepivacaine peak is about 1.6 minutes. The detector response is linear in the concentration of 10.060 to 30.180 mcg/mL, Y-intercept is -726.11468, Residual sum of square is 72329401.8155and slop is 54607.2822.The percentage assay of Mepivacaine is 99.9 %. The method is validated by determining its accuracy, precision and linearity.