Anti Bacterial and Phytochemical Screening of Trachyspermum Ammi – An In Vitro Approach
Journal: International Research Journal of Pharmacy and Medical Sciences (IRJPMS) (Vol.1, No. 4)Publication Date: 2018-07-10
Authors : Mohammed Sayeed Jonah Ratna Paul Patchigalla Revathi Oggu Rupa Ravali Ponna Srinivas Sri Navya Pajjuru Vasudha Bakshi Narender Boggula;
Page : 40-45
Keywords : Trachyspermum ammi; ajwain; broth dilution; cup plate method; phytochemicals; flavonoids; anti- bacterial activity;
Abstract
Treatment with medicinal plants is considered very safe as there is no or minimal side effects. Trachyspermum ammi is a folklore medicinal plant. The roots, seeds and leaves are used in traditional & folklore Medicine. The study was carried out to ascertain the anti bacterial properties present in ethanol extract of dried scale leaves of Trachyspermum ammi. The Anti bacterial testing of leaves extract Trachyspermum ammi was evaluated by Cup Plate Method and Minimum Inhibitory Concentration (MIC) by Broth Dilution Method using gram positive bacteria like Staphylococcus aureus, Bacillus subtilis, gram negative bacteria like Escherichia coli, Klebsiella pneumoniae. The potent activity was observed at 300µg/ml and other concentrations also shown the as well. The extract was more active against Gram negative bacteria such as Klebsiella and E. coli and did not show the marked activity on Gram positive bacteria such as Staphylococcus. It is considered as a valuable source of natural products for development of medicines against various diseases
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