SYNTHESIS AND CHARACTERIZATION OF POLY(D,L-LACTIC-CO-GLYCOLIC)ACID MICROPARTICLES LOADED BY DIPHTHERIA TOXOID

The aim of the study was to synthesize poly(D,L-lactide-co-glycolide) particles — PLGA and obtain their complexes with a non-toxic diphtheria toxin recombinant fragment subunit B fused with enhanced green fluorescent protein EGFP-SubB; to characterize the main physical and chemical properties of the resulting complexes. Two types of the diptheria toxoid loaded PLGA microparticles were obtained: particles with immobilized diphtheria toxoid (PLGA 1) and particles with encapsulated (PLGA) diphtheria toxoid (PLGA 2). Micropartcles obtaines were characterized for antigen loading, particle size, polydispersity index and in vitro antigen release.