EVALUATION FOR CYTOTOXIC POTENTIAL OF LEAF EXTRACT OF CALOTROPIS GIGANTIA

In the present study we have utilized the BSLB model (Brine shrimp lethality bioassay) and Allium cepa root meristem (ACRM) models to evaluate the cytotoxic activity of ethanolic leaf extract of c. gigantia. In BSLB model LC50 value of leaf extract was found to be 67.25 µg/ml while LC50 value of 5-flurouracil was found to be 66.01 µg/ml extract produced dose dependent growth inhibition. In ACRM model incubation of onion bulbs in different concentration of extract produced a growth retarding effect that was associated with a decreased in root number. Standard cytotoxic drug 5-flurouracil and non- cytotoxic drugs aspirin served as controls like 5-flurouracil. Ethanolic leaf extract significantly inhibited the growth of roots. In ACRM model % inhibition of root growth in leaf extract (ethanolic) after 48hrs was found to be 60.64% (1mg/ml), 72.35% (10mg/ml) and after 96hrs was found to be 85.85% (1mg/ml), 98.31% (10mg/ml) % inhibition of root growth in 5-flurouracil after 48hrs was found to be 63.83% (1mg/ml), 85.106% (10mg/ml) and after 96hrs was found to be 73.46% (1mg/ml), 96.46% (10mg/ml).