Protective association of IKAP T3214A polymorphism with asthma in a North Indian population: a case-control study

IKAP participates in activation of NF-κB and activated NF-κB is associated with over production of various inflammatory cytokines, chemokines and eotaxin genes in asthma. Genotyping of IκB kinase complex associated protein (IKAP) G2295A, T3214A and C3473T polymorphisms were carried out in a total of 964 individuals (483 healthy controls and 481 asthma patients) from a North Indian population using the PCR-RFLP method. Statistical analysis revealed a significant protection from asthma in TA genotype (OR=0.75, p=0.046) and combination of TA+TT genotype (OR=0.76, p=0.043) of T3214A polymorphism. Some asthma phenotypes were also found to be protective with this polymorphism. However, no polymorphism was observed in G2295A and non-significant association was found for C3473T polymorphism. Haplotype analysis of T3214A and C3473T polymorphisms found TA and TT to be significantly associated with asthma (p=0.016 and 0.012). The present study concludes that T3214A polymorphism confers protection from asthma among the studied North Indian population.