MORPHOFUNCTIONAL STATEOFKIDNEYS OF RATSAFTER INJECTIONOFPLACENTAL CRYOEXTRACT IN HEYMANN NEPHRITIS

The studying of immune mechanism of kidney damage, under the model of Heymann nephritis (HN), causing vascular changes in glomeruli, their morphological and morphometric changes are of considerable interest. Objective: To study the effect of injection of allogeneic placenta cryoextract (APC) on renal function and morpho-metric markers in experimental HN. Materials and methods: 35 non-linear male rats 4 months old were divided into 3 groups: 1 - intact; 2 - animals with a HN model; 3 - animals with a model HN, which on 28th day after immunization received 3 times per week ACP intramuscularly. Animals 2nd and 3rd groups were taken out of the experiment at 45 and 60 days. Morphological and bio-chemical markers of condition of the kidneys at all stages of development HN and after the injection of ACP were studied by light and transmission electron microscopy. Results and discussion: In the blood of animals 2 and 3 groups on the 28th day the number of circulating immune complexes (CIC) and immune deposits on the glomerular basal membrane were increased, which was accompanied by disturbance of their structure and changes in renal excretory function. Revealed morphometric differences in the size of the glomeruli in the NH dynamics. After 60 days in tissue of kidney of experimental animals identified signs of focal mesangial proliferative glomerulonephritis, both after APC injection, and without it. With the injection of APC deposition of immune complexes was not found. Conclusions: In case of HN to 28th days increased amount in blood of CIC, increased deposition of immune deposits in the glomerular basement membrane of vessels, resulted to vessels narrowing and mezangiocytes proliferation and was accompanied by renal dysfunction. Injection of APC in case of NC can stop its progression, and eliminate disturbance of renal blood flow in cortical glomeruli.