Isolation and Characterization of Gibberellic Acid 3 Producing Fusarium Sp. from Belgaum Agriculture Land Andits Impact on Green Pea and Rice Growth Promotion

Worldwide ultimate aim of any agriculture sector or farmer is to take maximum yield. Sufficient supply of nutrients and fertilizer are not able to give maximum yield. There are numerous factors which are responsible for low yield, among that one is the environment stress or the unstable climate conditions. To increase the yield there are numerous approaches like use of genetically modifiedcrops, but in India it is controversial approach and another approach is the use of multifunctional plant hormone like Gibberellic Acid3 (GA3).This research mainly involves the production of GA3 from fungal species and to apply it on crop plants. Fusarium species were isolated from Belgaum agriculture soil and screened for GA3 production under submerged fermentation. Strain showing maximum GA3 yield (strain M104) was chosen to study the effect of various parameters on GA3 production, like incubation time (1-12 days), initial pH (5.0-8.0), incubation temperature (20-50°C), pH (5.0 - 8.0), and carbon and nitrogen sources. The maximum production of GA3 was observed on day 8 at 30°C, and pH 5.5 with glucose and ammonium chloride as good carbon and nitrogen sources, respectively. After optimization, a Keywords: Fusarium sp; Isolation; Gibberellic Acid; Optimization; Submerged Fermentation; Crop Plants 5.56-increase in GA3 production was observed. The GA3 production was confirmed bythin layer chromatography. The GA3 crude extract obtained using submerged fermentation was then used to study its effect on germination and growth of green pea plant and paddy crops. It was observed that GA3 treated crops showed uniform growth and they were taller than non-treated plants, suggesting its application in increasing the crop plant harvests.