EVALUATION OF AQUEOUS EXTRACT OF CLERODENDRUM COLEBROOKIANUM ON D-FRUCTOSE INDUCED TYPE 2 DIABETES AND IN VIVO ANTI-OXIDANT STUDY ON EXPERIMENTAL ANIMALS

Background Present study was undertaken to investigate the antihyperglycemic, hypolipidemicand in vivoanti-oxidant effects of aqueous extract of Clerodendrumcolebrookianum (AECC) in experimental diabeticrats. MATERIALS AND METHODS The above experiment was carried out with C.colebrookianum extract@ 100 and 300 mg/kg, p.o. for a period of 14 days in diabetic rats. During the experimental period, blood was collected from the fasted rats to determine the blood glucose and lipid profile. Body weight of the animals was recorded on 0th, 7th and 14th day. After the treatment period, lipid peroxidation (LPO), reduced glutathione (GSH), glutathione –S-transferase (GST), catalase (CAT) and superoxide dismutase (SOD) were also assayed. RESULTS Diabetic control rats showed a significant elevation in fasting blood glucose on successive days of the experiment, which was maintained over a period of two weeks.After 14 days treatment with AECC (100 and 300 mg/kg, p.o.) and standard drug (Glibenclamide @ 1 mg/kg), a significant (P<0.01) decrease in the blood glucose as well as plasma and liver total cholesterol, triglycerides and phospholipids was observed. Besides, anti-oxidant properties of AECC was also elucidated in this experiment and found that administration of AECC to the diabetic rats caused significant (P< 0.01; P< 0.05) reduction of liver enzymes viz LPO, GSH, SOD, CAT & GST. CONCLUSIONS Our results demonstrated that AECC with its antidiabetic, hypolipidemic and antioxidant properties could be a potential herbal medicine in treating diabetes and other related complications.