Peptide Mass Printing of Hemocyanin Protein of Spider, Hippasa Agelenoides From South India Using Maldi-TOF MS

Abstract: In proteomics, MALDI is used for the rapid identification of proteins isolated by using SDS-PAGE, size exclusion chromatography, affinity chromatography, strong/weak ion exchange, isotope coded protein labeling (ICPL), and two-dimensional gel electrophoresis. The simplicity, robustness and sensitivity of MALDI have made it a fundamental technique of proteomics. Here we report a high throughput protein identification of hemocyanin protein from the spider Hippasa agelenoides by MALDI TOF MS, peptide mass fingerprinting.