Anti-aging effects of the proteins from artemia extract on human fibroblasts cell proliferation and collagen expression in induced aging conditions

Researches on the factors that inhibit senescence are attractive in health industries. Artemia extract uses in cosmetic and sunlight protecting products. The aim of this work was to study the effects of Artemia extract on the cell proliferation, senescence and collagen synthesis by human fibroblasts in normal and aging induced conditions. Artemia extract was extracted from napauliies and partially purified in 7 fractions. The human foreskin fibroblasts (normal and H2 O2 stressed) were treated by crude and/or fractions in a dose dependent manner for 96 h. Cell counting, MTT and BrdU assays indicated that the fractions 4,6,7 induce cell proliferation. Treatment of cells by these fractions resulted in the reduction of senescence-associated ß-Galactosidase activity up to 50% in comparison to H2 O2 treated control cells. The highest collagen type I mRNA expression was observed in fractions 5 and 7. The mRNA level increased in comparison with untreated and house keeping gene (β-actin) mRNA level. Percent of collagen type I expression to β-actin was 2.19 and 1.31 fold in cells which treated by fractions 5 and 7. Artemia extracts induce fibroblast proliferation, collagen synthesis and reduce cell senescence. These finding support the anti-aging effect of this product for probable application in the future.