DYNAMICS OF BIOCHEMICAL PARAMETERS OF BLOOD OF PREPUBESCENT AGE RATS KEPT ON CARBOHYDRATE TYPE OF FOOD WITH ADDITIONAL CORRECTION OF THE MICROBIOME OF DIGESTIVE TRACT BY BIOTIC

Aim. To study influence of carbohydrate-rich type of nutrition on changes of the biochemical parameters of blood serum, on qualitative and quantitative structure of microbiota of the large intestine and feces of prepubescent age rats and to estimate possibilities of additional correction with probiotic drug «Normobakt». Materials and Methods. Experimental model was created using rats of Vistar line. The first group of laboratory animals (n=10) received carbohydrate-rich food (77% of the general diet) within 7 weeks. In the second group of experimental animals (n=10) correction was used with «Normobakt» preparation within 2 weeks. The control group (n=10) was given the balanced diet. Biochemical parameters of blood serum were estimated using the automatic biochemical SA-400 analyzer (Japan) with liquid stable diagnostic kits (Diasys, Germany). Bacteriological examination of the large intestine and feces was conducted using nutrient media. Results. With carbohydrate-rich diet a reliable increase in glucose and aspartate aminotransferase and reduction in the total protein in blood were noted. No reliable changes in parameters of lipid profile were found. In large intestinal microflora and feces, rapid growth of Candida fungi was noted with simultaneous reduction of Bifidobacterium spp. in the large intestine and of Bifidobacterium spp. and Lactobacillus spp. in feces. Correction by the medical preparation «Normobakt» led to a reliable reduction in the level of glucose and AST in blood serum. In the microbiota of the large intestine and feces the level of Bifidobacterium spp. and Lactobacillus spp. reliably exceeded parameters of the control group, while growth of opportunistic microflora reduced to zero. Conclusion. Thus, carbohydrate-rich type of nutrition in the prepubescent period leads to significant changes in the biochemical parameters of blood, as well as to disorders in the structure of microbiota. Application of the medical preparation «Normobakt» is an effective strategy for correction of microbiome and of metabolic disorders induced by unbalanced nutrition.