Aneuploidy of Urethane in Mouse Bone Marrow Cells and Potential Recovery with Lupin Water Extract

The incidence of in vivo urethane-induced chromosomal aberrations, sister chromatid exchanges (SCEs) and aneuploidy was examined in male mice. Single oral administration by gavage with urethane (0.5 and 1 g/kg) caused a significant increase in chromosomal aberrations in bone marrow and spermatocyte cells, and statistical significant in SCE induction. The clastogenic effect observed was dose- and time- dependent. Aneuploidy was observed clearly with the high dose recording a significant value. Administration of lupin water extract at 5000 ppm/mice/day (added with the drinking water) reduced the frequency of chromosomal aberrations, but still at the significant values (P, 0.001) while that administration of lupin extract elevated the aneuploidy induced with urethane. It can be concluded that urethane is a strong clastogenic and weak aneugenic agent when administered orally and administration of lupin water extract can be elevateing the aneugenic property of urethane.