Embryogenic Callus Induction from Mature Embryos of Sorghum Genotypes

Sorghum bicolor is the fifth most important cereal crop in the world. Sorghum is considered to be one of the most recalcitrant species among the cereals for in vitro response and genetic transformation. In this study four sorghum genotypes were examined for their ability for callus induction. The cultures were initiated from mature embryos. The highest number of somatic embryos was obtained on MS medium supplemented with 2mgl-1 2, 4 D. Highest frequency of embryogenic callus formation was observed in the genotype Wad Ahmed.