Bacteriological Study of Coagulase Positive Staphylococci from Urine in Some Akoko Communities, Ondo State, Nigeria

Staphylococci which can be normal skin flora as well as aetiologic agent was examined for their clinical implications from urine sources during the study. The occurrence of staphylococcal species in urine sources of some people in Akoko communities between age group 5 to 55 years was determined. 50 sample sources were screened for this purpose. The total viable bacterial count of urine sample sources range from 0, 1 x 103 cfu/ml and 4 x 103 cfu/ml from Ikun-Akoko, Supare community, Iwaro-Akoko as well as Akungba-Akoko respectively to high microbial load of 50 x 106 cfu/ml from Supare-Akoko. The bacterial isolates were identified using standard microbiological techniques including biochemical characteristics in which 23 (46%) were coagulase positive and 25 (50%) were coagulase negative and 2 (4%) show no growth on Mannitol Salt Agar. The 48 isolates obtained for this study were all Gram positive cocci (Bacteria). The Staphylococcal species obtained were grouped as C.P.S a 1:- Coagulase positive Staphylococcus aureus group 1, C.P.S.a 2:- Coagulase positive Staphylococcus aureus group 2, C.NS 1; Coagulase negative Staphylococcus group 1, C.N.S 2: Coagulase negative Staphylococcus group 2 and C.N.S 3: Coagulase negative Staphylococcus group 3. Some of the isolates tested show multiple antibiotic resistance which is significant clinically in case of treatment of infections in immunocompromised persons. The physico-chemical properties of urine samples collected from the Akoko communities studied was determined using Combi 9 strip/kit. This study will help to generate a database for health management purposes with the aim of controlling Staphylococcus infections.