COMPARATIVE CHARACTERISTICS OF THE LIVER MORPHOFUNCTIONAL STATE OF THE FETUSES FROM MOTH-ERS WITH SUBACUTE INFECTIOUS IN-FLAMMATION OF THE ABDOMINAL CAVITY CAUSED BY KLEBSIELLAE PNEUMONIA AND THE FETUSES WITH CHRONIC PRENATAL HYPOXIA

The aim of the study is to investigate peculiarities of the liver morphofunctional state of the fetuses from mothers with subacute infectious inflammation of the abdominal cavity caused by Klebsiella pneumoniae in the experiment. For morphological studies, we used fetal liver of the rats, which were divided into 3 groups: control (ContG), experimental (ExG) and a comparison group (CompG). ContG (n = 9) – fetuses from pregnant female rats with physiologically occurring pregnancy, ExG (n = 10) – fetuses from pregnant female rats with a subacute infectious inflammatory process in the abdominal cavity, modeled using reference strains of Klebsiella pneumoniae (NCTC 5055), CompG (n = 7) – fetuses with chronic prenatal hypoxia (CPH). The taking, fixation of the material and the production of paraffin blocks were carried out in accordance with generally accepted pro-cedures. Serial cross sections were stained with hematoxylin and eosin. Interstitial collagens were detected using indirect Coons method in Brosman modification with monoclonal antibodies (MAbs) to collagens I, III (Novocastra Laboratories company Ltd, UK). The preparations were studied with fluorescent microscope "Axioskor 40" (Carl Zeiss, Germany). The area and volumes of the nucleus, cytoplasm and whole volume of the hepatocytes, the nuclear-cytoplasmic index (NCI) and the density of the location of the hepatocytes in 1mm2 were determined with video microscopic morphometry with using of the program "Olympus DP-soft version 3.1", Microsoft Excel. In preparations treated with MAbs was determined the fluorescence intensity in conditional units of luminescence. All data were processed by methods of math-ematical statistics using variation and correlation analysis. The Mann-Whitney-Wilcoxon test was used to determine the reliability of the results, the Spearman correlation coefficient was used to perform the correlation analysis. The collagenization process was more pronounced in the ExG. The obtained data indicate that in rats from mothers with prolonged infection caused by Klebsiella pneumoniae and in fetuses with CPH in the liver the same changes in the form of central pleural veins and sinusoids, violations of the lobular structure of the hepatic lobe, dystrophy and necrosis of hepatocytes with regenerative increasing in the proliferative activity of hepatic cells, the development of their compensatory hypertrophy and the stimulation of the synthesis of interstitial collagens are observed. In the fetuses from mothers with prolonged Klebsiella infection in comparison with fetuses with CPH, the degree of severity of discirculatory changes, alterative processes in the parenchymal component of the liver increases, which is accompanied by a decrease in the functional activity of hepatocytes and increased sclerotic changes in the stromal component of the organ, which can lead to disruption of the adaptive capacity of the liver further ontogeny.