EXPERIMENTAL RATIONALE OF THE USE OF MINERAL WATERS OF MORSHYN RESORT IN TREATMENT OF PATIENTS WITH NON-ALCOHOLIC FATTY LIVER DISEASE

Оbjective : to check the efficiency of internal use of mineral diluted water of Ninivsky well № 3-k (Morshyn resort) with a total mineralization of 3-4 g/dm3 and 6,5-7,5 g /dm3 on the condition of rats with a model of non-alcoholic fatty disease liver to substantiate the possibility of its use in the complex treatment of patients with NAFLD (non-alcohol fatty liver disease). Materials and methods. The experiment was performed on 60 white rats, females with body weight 180,0-200,0 g. The animals according to the tasks of the work were ranked in 3 groups. Group I - 20 rats, which was modelled with NAFLD. Group II (20 rats) which, on the background of the reproduction of the pathological model from 15 to 30 days of the experiment, received the MW of the well № 3-K (sulfate, chloride-sulfate-sodium) diluted by MW (source № 4, Morshyn resort) of Ninivsky well of Lviv region to total mineralization 3,0-4,0 g/dm3. Group III (20 rats), which on the background of reproduction of the pathological model from 15 to 30 days of the experiment received the same MW diluted to a total mineralization of 6,5-7.5 g/dm3. Upon completion of the study, the animals were withdrawn from the experiment by decapitation under anesthesia. A small part of liver (1 cm3) was taken from the animals. One slice was rinsed with the spirits of high concentration and put into celloidine. Histological cuts were made, which were stained with hematoxylin-eosin. Microscopic examination of the liver was performed on the sections obtained. The second slice was frozen with dry carbonic acid (- 70 º C), on the manufactured sections, histochemical reactions were performed on the determination of the activity of succinate dehydrogenase (SDH) and lactate dehydrogenase (LDH) in the standard units of optical density (unit volume density) by Loyd. The statistical processing of the data obtained in the series of experiments was carried out using the programs of medical and biological researches Statistica and Exel. For all means of processing, the reliable changes were those that were within the probability of the Student tables P<0,05 Results and discussion. As the result of the experimental studies, an improvement in the morphological pattern of the liver was demonstrated under the influence of the mineral water drinking course. This was determined by the sharp weakening of the disappearance of signs of fatty infiltration, the reduction of signs of hepatocyte dystrophy and the restoration of uniform activity of enzymes over the entire incision of the lobe. At the same time, the strength of the positive effect of the use of MW increased in direct proportion to the total mineralization of water (osmolarity). Conclusions. The obtained results substantiate the expediency of using of mineral water from Morshyn Health Resort in clinical trials for the treatment and prevention of non-alcoholic fatty liver disease.