STAPHYLOCOCCUS AUREUS ANDESCHERICHIA COLICAUSING SUBCLINICAL MASTITIS IN SHEEP AND GOATS

Mastitis is one of the most serious economic problems in all dairy sheep and goats flocks. Examination of 110 milk samples collected from apparently healthy sheep and goats using the California mastitis test (CMT) showed that 49 (44.5%) were CMT positive. Standard methods for isolation and identification of S.aureusand E. coli could identify 3 and 9 strains respectively. Five different serogroups weresuccessfully identified among 9 E. coli strains, the serogroups were O26 (33.3%), O1 (22.2%), O114 (22.2%), O148 (11.1%) and finally O125 (11.1%). The antimicrobial susceptibility results for erythromycin and Cefoxitin revealed that all E.coli isolates were resistant to erythromycin (100%) followed by high frequencies of resistance observed to Cefoxitin 8 isolates (88.9%). All S.aureus isolates wereCefoxitinresistance while no resistance pattern was detected to erythromycin. The Phylogenetic grouping of E.coli by triplex PCR using two genes (chuA and yjaA) and an anonymous DNA fragment tspE4C2 indicated that 5/6 were the B2 group and 1/6 was A group. The genotyping identification using nuccan identify 2 strains only of S.aureus isolates considered it of low identification power than use the traditional biochemical tests and Vitek 2 System identification in vitro.