Novel Synthesis and Characterization of Silver Nanoparticles from Leaf Aqueous Extract of Aloe Vera and their Anti-Microbial Activity

The novel approach has been carried for the green synthesis of silver Nano particle using leaf extract of Aloe Vera and silver nitrate solution. The synthesis silver nanoparticles were characterized by using various instrumental techniques including (UV-VIS) Spectrophotometry, XRD Spectrophotometry, DRS Spectrophotometry, Scanning Electron Microscopy (SEM), and Fourier Transform Infrared Spectroscopy (FTIR).The EM) showed the formation of silver nano particle with an average size of 87.7 nm. The synthesized nanoparticles are found to be highly effective against different human pathogenic bacterial species including gram-negative Pseudomonas aeruginosa, salmonella, and Escherichia coli and gram-positive bacteria like Staphylococcus aureus by using different concentration of silver stock solution i-e 20 µg/ml, 40 µg/ml, 60 µg/ml, 80 µg/ml, and 100 µg/ml.Results revealed that the synthesise silver nano particle exhibit excellent zone of inhibition against Staphylococcus aureus 04mm, 09mm,13mm and 17mm and 19mm using Gentamicin as standard drug but did not show exhibit inhibitory effects against Escherichia coli,Pseudomonas aeruginosa,Salmonella. Thus their excellent antibacterial activity against resistant strains suggests that warrant further optimization as novel anti-infective chemotherapies.