Separation and Identification of Many Natural Products from Emblicaofficinalis Fruits and Study of their Antibacterial Activity

The active constituents were separated from Emblicaofficinalis fruit, using column chromatography (CC) with solvent system of (pet.ether:ethyl acetate; 5:1) from petroleum ether extract (A1) and solvent system (chloroform:Methanol; 10:1) from Ethanol extract (A2). Saponification process was carried out on (A1F2) to separate many fatty acids and identification of five fatty acids (Heptanoic, Octanoic, Lauric, Palmitic and also cis& trans Oleic acids) using GLC-analysis. Moreover, acid hydrolysis was also carried out on (A2F2), to separate many free poles of phenolic compounds, such as (Hydroquinone and Gallic acid) were identified using HPLC-analysis. The active separated compounds under study showed different effects against the micro-organisms (m.o), using disc diffusion method turbidity.