In vitro and In Situ Evaluationof the Efficacy and Efficiency of Products to Clean, Disinfection and Sterilization Process in Veterinary Environmental

Pathogen microorganisms resistance presence in surgical instrument and places where process are performed are the most common right now, this kind of topics are very important and robust on humans, for this reason, the study of bacterial resistance is popular, when is compared with livestock areas, the issue is the same, but less study the trouble, this represent a big annual economic loss, as has been reported different livestock environments that the contamination levels can achieve 82% to periods of until 5 years, this indicates a need for research, for that reason the objective of this study was to evaluate the effectiveness and efficiency of products for the cleaning, disinfection and sterilization process in veterinary environments, the target in this research was to develop different methodologies, in order to carry out previous microbiological identifications, bioassays with four products: 1) monoenzymatic detergent (protease) and quaternary ammonium, 2) high-level disinfectant detergent based on potentiated glutaraldehyde (0.17%) and quaternary ammonium, 3) broad-area disinfectant detergent with quaternary ammonium grapefruit fragrance and 4) disinfectant detergent of furnishings based on quaternary ammonium. In addition, cytotoxic, extracellular and In situ activities were determined, from which it was possible to conclude that all evaluated products have bactericidal, fungicidal and virucidal activity, without affecting the cells or tissues where the microorganisms were found.