The Determination of AFM1 and AFM2 in the Crude Milk by HPLC in the Province of Diyala

The residues of aflatoxin M1 and aflatoxin M2 may be present in the curde milk and milk products, which may cause health problems for the consumer, Crude milk collected from dairy farms in the Diyala Province, were isolated and examined for aflatoxin M1 (AFM1) and aflatoxin M2 (AFM2). In this study 100 samples of milk were collected during the period between September to December 2017, the results revealed the presence of 12 isolate of fungal spp. And they are (Aspergillus flavus (17%), A. Terrace (7%), A. niger( 48%), A. fumigates(6%), A. ochraceus (4%), Penicillum spp. ( 10%), Candidia spp.(31%), Rhodotorule spp.(23%), Fuserum(2%), Cladosporium (2%), Risobes spp. (4%) and Geotricum candidium (2%)). Aflatoxins are naturally occurring mycotoxins that are produced by Aspergillus flavus , This study also revealed the analysis of 15samples by HPLC to detect the concentration of AFM1 and AFM2 in milk samples which produced positive results of Aspergillus flavus where the results show the concentration of AFM1 vary from (0.26-0.00076) and for AFM2 vary from (0.1-0.00009) in the samples of the Crude milk.