THE EFFECT OF GLUCONATES 3D-METALS ON THE BIOCHEMICAL INDICATORS OF OXIDATIVE AND IMMUNE HOMEOSTASIS IN EXPERIMENTAL IMMUNODEFICIENCY

Aim. Analysis of relationships between biochemical parameters of oxidant-antioxidant system and phagocytic activity, synthesis of cytokines, antibodies in experimental immunodeficiency, as well as the effect of 3d-metal compounds with gluconic acid on them. Materials and Methods. 3d-metal gluconates (Mn, Fe, Co, Cu, Zn) synthesized in the Ufa Institute of Chemistry UFRC RAS were administered orally to laboratory mice at a concentration of 10-2 mol/l for two weeks, starting from the second day after the injection of cyclophosphamide (50 mg/kg) to induce experimental immunodeficiency. The activity of superoxide dismutase (SOD), catalase (CT), glutathione peroxidase (GPO) and glutathione transferase (GT), the content of TBA-active products (TBA-AP) and oxidatively modified proteins by the level of spontaneous and induced carbonylation (CP sp, CP in) were determined in liver homogenate. Serum levels of IgG, their complexes with the first subcomponent of complement C1q and cytokines (IL-1β, IL-6, IFN-γ, and TNF-α) were determined (test kits FSUI IHPB FMBA of Russia, St. Petersburg). Blood stabilized with heparin was determined indices of the absorptive and metabolic activity of phagocytes: the phagocytic number (FN), phagocytic index (PI), phagocytic integral index (PII), indicators of spontaneous and induced NST-test (NST-SP and NST-IN), the average cytochemical coefficient (CCC) and stimulation index (SI). Correlation analysis between oxidant and immune homeostasis was performed using the program «Statistica 10.0», determining the Pearson coefficient. The values at pResults. Between indicators of oxidant-antioxidant and immune systems of intact animals revealed correlation of high strength: TBA-AP, CB sp, CP in, CT and GPO with FI and NST-IN; NST-SP – GPO and GT; IgG – SOD; IL-6-all (except GT), TNF-α – all except SOD and GT; IFN-γ – GT. When immunodeficiency was induced, there were significantly fewer reliable links between the systems. The introduction of 3d-metal gluconates led to the restoration of lost relationships, which indicated the activation of metabolic processes in phagocytes and cytokine production. The greatest effect was observed under the action of zinc and copper gluconates. Conclusion. One of the possible mechanisms of immunomodulatory action of combinations of 3d-metals with gluconic acid is their ability to influence the oxidant-antioxidant system.