Potensi Brazilein Potensi Brazilein dari Kayu Secang (Caesalpinia sappan L.) Sebagai Agen Depigmentasi Kulit Secara In Silico

Hyperpigmentation and skin darkened occur due to oversynthesis of melanin as result of increased activity of tyrosinase enzyme. Brazilein is the major content which found in kayu secang (Caesalpinia Sappan L.) which is empirically able to brighten the skin. The aim of this study was to determine the potential of brazilein as a skin depigmentation agent against protein target tyrosinase enzyme with a in silico test compared with hydroquinone and ascorbic acid. The stages of this study were optimization of brazilein, hydroquinone and ascorbic acid 3D structure using Hyperchem 8, Preparation of protein targets using Chimera 1.10.1, validation of molecular docking and Docking of brazilein, hydroquinone and ascorbic acid on protein target using Autodock 4.2. The result of molecular docking was bond energy. Analysis was done by comparing the results of docking brazilein on protein targets with result of docking hydroquinone and ascorbic acid on protein target. The bond energy values between brazilein, hydroquinone and ascorbic acid on protein target respectively -6,70, -3,78 and -3,84 kcal/mol. The value of brazilein bond energy was less than hydroquinone and ascorbic acid in the protein target. The lower of ligand bonding energy with the protein target, then the stronger of bond formed between ligand with the protein target and the affinity of the ligand against protein target was directly proportional to the strength and stability of the bond.This comparison of bond energy values indicates that brazilein has a greater potential as a skin depigmentation agent compared with hydroquinone and ascorbic acid with a mechanism of inhibiting tyrosinase enzyme with in silico test by molecular docking method