GENETIC VARIABILITY BETWEEN THE POPULATIONS OF BOSWELLIA SERRATA ROXB. BY USING RAPD PRIMERS
Journal: International Journal of Engineering Sciences & Research Technology (IJESRT) (Vol.8, No. 1)Publication Date: 2019-01-30
Authors : Shashank Mahesh Deepak Mishra; Pramod Kumar;
Page : 163-168
Keywords : Boswellia serrata Roxb.; RAPD; PIC; phylogeny.;
Abstract
Boswellia is the gummy resin plant of the boswellia tree. It has the family Burseraceae. It's local name are Guggal, Salai Guggal, Sallaki. Boswellia Gum Resin is the most important component of Boswellia Serrata tree. It has the indigenous origin to India and being applied over hundred years since Ayurvedic times by the doctors. In this study, three populations having 57 accessions (Jabalpur, Mandla and Balaghat) were selected to find genetic diversity between and within the individuals. The extracted genomic DNAs obtained the purity from 1.5-1.7. Three highly polymorphic RAPD (Random amplified polymorphic DNA) primers were selected randomly to amplify these genomic DNA samples. Here, the primer OPAW-01 showed the minimum PIC 0.93 and the average of PIC obtain from three primer was 0.83 it means the heir amount of PIC showed that there is a huge genetic difference between groups are individual and the gene diversity which is also maximum in primer OPAW-01 (0.93) showed that there genetic character and it may be within the groups and individual this may be occurs due to the climates change are variation at gene or allele level
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