In-Vitro Interaction of Cefpodoximeproxetil with Different Essential Metal, Antacids and Investigation of Antimicrobial Activity

The research work comprises of interaction studies of cefpodoximeproxetil with essential metals and investigation of antimicrobial activity of cefpodoximeproxetil. Cefpodoximeproxetil is included among the cephalosporins drug class which is active against a wide range of gram positive and negative bacteria.Since the presence of compelling ligand may affect the bioavailability of a metal in the blood or tissues, therefore in order to study the probable interaction of cefpodoximeproxetil with essential and trace elements present in the body. Cefpodoximeproxetil has been interacted with Mg (OH)2 (magnesium hydroxide), CaCO3 (calcium carbonate) antacids and Zn metal salts in vitro. All the reaction conditions were simulated to natural environment. Also the antimicrobial activity of the drug and the complexes were determined. There is a effect of pH on drug metal complexation. It has observed that cefpodoximeproxetil interacts with metal on a PH 7.4, the stability constant of these complexes were determined in order to evaluate their possible in vivo implications. In order to investigate the number of metal &antacid involved in the complexion with cefpodoximeproxetil complexes were elucidated by exploiting various spectrophotometric methods. The ultraviolet studies of these complexes were carried out and compared. Here due to interaction of both cefpodoximeproxetil with metal & antacid, it is shown that the standard absorbance of cefpodoximeproxetil is quite different from the absorbance of mixture of both drugmetal & drug-antacid. Otherwise, in antimicrobial study we have seen the same thing that is due to their interaction the antimicrobial effect or activity of cefpodoximeproxetil is reduced. The standard zone of inhibition of cefpodoximeproxetil is 17mm but since cefpodoximeproxetil is interacted with metal[ZnSO4 .7H2O] & antacid [Mg(OH)2], [CaCO3] it is reduced into 14mm, 10mm, 13mm.