Immunomodulation and Antihypertensity Study of Green Tea Extracts Induced with Hydrocortisone by Using Animal Model

Green tea and their constitutents play an important role in defense against pathogens to the modulation of immune system. Antioxidant and other substances in green tea helps to protect the heart and blood vessels. Antimicobial activity of green tea extract was determined by disc diffusion method the results shown the maximum zone of inhibition of green tea extract for P. aeruginosa. While the inhibition zone with 13mm was observed with P. fluorescence followed by E. coli and Shigella flexneri. The present study green tea extract was given to mice for four weeks to assess the immune response by measuring phagocytic cells and antibody production. Administration of EGTE 200 mg/kg exhibited 2.76 ± 0.22. The result revealed EGTE from green tea enhance multiplication of leukocytes. Studies related to polymorphic cells observed as differencial count expressed 42% cells/cubic mm meadiated with hydrocortisone alone. The lymphocytes was found to be 58% cells/cubic mm enhanced with green tea and hydrocortisone combination. The effect of serum biomarkers SGOT and SGPT observed for 15 days with oral administraion of green tea revealed body weight was decreased after the intake of green tea. Both SGOT and SGPT was found to increase. A remarkable difference was observed with the marker enzyme SGPT when hydrocotisone treated mice revealed 59 U/ml. Besides another marker enzyme SGPT revealed the serum level of 40 U/ml. Similar results were obtained for triglycerides. The effects induced by hydrocortisone was compensated by the treatment with the extract of green tea as special diet. Studies pertaining to the effect of EGTE on hypersensitivity mediated ractions (IgE) in mice was measured by ELIZA which suggest EGTE an ingrediant from green tea extract might be useful in counter acting allergic responses. The study was further extended to determine hypertensive effect of green tea supplemented with egg led to a remarkable increase in total cholesterol. The increased activity of hepatic serum biomarkers SGOT and SGPT perhaps a well diagnostic indicators of hepatic injury.