COMPLEX ANALYSIS OF CLINICAL-MORPHOLOGICAL AND MOLECULAR GENETIC MARKERS IN PATIENTS WITH CHRONIC DERMATOSIS ASSOCIATED WITH FACIAL SKIN ATROPHY

Background. The ralationship between skin structural alterations and activity variations of genes controlling collagen and elastin synthesis in patients with chronic dermatosis associated with facial skin atrophy is not well known. Objective. To clarify associations between clinical-morphological and molecular genetic markers in skin biopsy samples by using complex analysis. Material and methods. Skin punch-biopsies with the diameter from 1 to 4 mm were used as research material. Histological analysis was carried out for morphological features estimation; real-time PCR with reverse transcription was performed for evaluation of the gene expression level. Results. Morphological alterations in the skin of patients with chronic dermatosis are associated with the level of normalized expression of genes: gene СOL1A1 less than 100% and/or gene COL1A2 less than 200% and/or gene LOX less than 50%. Conclusions. Real-time PCR with reverse transcription can be used for objective estimation of the degree of skin alterations in patients with chronic dermatosis.